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Additionally, we are finalizing as proposed the incorporation of a provision at § 425.400(c)(1)(x)(C), specifying that primary care service codes used for purposes of assigning beneficiaries include a CPT code identified by CMS that directly replaces a CPT code specified at § 425.400(c)(1)(x)(A) or a HCPCS code specified at § 425.400(c)(1)(x)(B), when the assignment window or expanded window for assignment (as defined at § 425.20) for a benchmark or performance year includes any day on or after the effective date of the replacement code for payment purposes under Medicare FFS

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After incubations with various juices and preparations for 24 h at 37 °C, the viable cells in each well were determined using the Cell Counting Kit-8 (CCK-8, Enzo Life Sciences) according to the manufacturers’ instruction